- Obstetricians & Gynecologists, Gainesville - Ob-Gyn Doctors, Physicians & Surgeons (Obstetrics & Gynecology)
- Occupational Health Care Physicians & Surgeons, Gainesville - Occupational Medicine
- Occupational Health Care Professionals, Gainesville
- Off Road Vehicles, Gainesville
- Off Road Vehicles Parts & Accessories, Gainesville
- Office Buildings & Parks, Gainesville
- Office Equipment & Supplies Manufacturers, Gainesville
- Office Supplies, Equipment & Office Furniture Stores, Gainesville
- Oil Change & Lubrication, Gainesville - Auto Oil & Quick Lube
- Oil Industry & Oil Field Equipment & Services, Gainesville - Oil Companies
- Oil Petroleum Wholesale, Gainesville
- Oilfield Trucking, Gainesville
- Oils Industrial, Gainesville
- Oncologists, Gainesville - Cancer Doctors, Physicians & Surgeons (Oncology)
- Online Advertising, Gainesville - Internet Advertising
- Ophthalmologists, Gainesville - Eye Doctors, Physicians & Surgeons (Ophthalmology)
- Optical Stores - Eyeglasses, Lenses & Sunglasses, Gainesville
- Opticians, Gainesville
- Optometrists, Gainesville - Eye Doctors, Physicians & Surgeons (Optometry)
- Optometry Clinics, Gainesville - Eye Clinics
- Oral Surgeon, Gainesville - Dentists - Oral & Maxillofacial Surgeons
- Orchestras, Symphonies & Bands, Gainesville
- Oriental Restaurants, Gainesville
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- Ornamental Iron - Residential, Gainesville - Wrought Iron Work Supplies, Parts, Gates, Railings, Stairs, Spirals, Fence, etc
- Ornamental Metal Work, Gainesville - Steel, Iron, Bronze, etc
- Ornamental Nursery, Gainesville
- Ornamental Nursery Services, Gainesville
- Orthodontist, Gainesville - Teeth Straightening, Invisalign & Dental Braces
- Orthopedists, Gainesville - Orthopedic Surgery Doctors, Physicians & Surgeons
- Other Attorneys & Lawyers, Gainesville
- Other Attorneys & Lawyers, Gainesville
- Other Building Materials & Supplies, Gainesville
- Other Industrial Contractors, Gainesville
- Other Lawyers & Attorneys, Gainesville
- Otolaryngologists, Gainesville - Ear, Nose, Throat - ENT Doctors, Physicians & Surgeons (Otolaryngology)
- Outboard Motors Parts & Repair, Gainesville
- Outdoor & Lawn & Garden Equipment Repair & Service, Gainesville
- Outdoor Advertising & Billboard, Gainesville
- Outdoor Centers, Gainesville
- Outdoor Equipment & Accessories, Gainesville
- Outdoor Power Equipment Repair, Gainesville
- Outpatient Services, Gainesville - Outpatient Care
- Outsourcing Services, Gainesville
- Overhead Door, Gainesville
- Ownership Organizations, Gainesville
- Oxygen & Respiratory Equipment & Supplies, Gainesville - Oxygen Concentrator, Tank, Machine, etc
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